Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rat testis tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue, human skin tissue, human lung tissue, human ovary tissue, human breast cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 23 publications below |
| IHC | See 5 publications below |
| IF | See 11 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10676-1-AP targets FABP3 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1069 Product name: Recombinant human FABP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-133 aa of BC007021 Sequence: MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA 相同性解析による交差性が予測される生物種 |
| Full Name | fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC007021 |
| Gene Symbol | FABP3 |
| Gene ID (NCBI) | 2170 |
| RRID | AB_2102309 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05413 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FABP3 antibody 10676-1-AP | Download protocol |
| IHC protocol for FABP3 antibody 10676-1-AP | Download protocol |
| WB protocol for FABP3 antibody 10676-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab An integrated multi-omics analysis reveals osteokines involved in global regulation | ||
Cell Stem Cell Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development | ||
Bone Res Pak4-mediated crosstalk between necroptotic macrophages and tendon stem/progenitor cells contributes to traumatic heterotopic ossification formation. | ||
ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy | ||
Cell Discov Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals. | ||
Adv Sci (Weinh) BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation. |