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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F4/80 Recombinant antibody

F4/80 Recombinant Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2P18

Cat no : 81668-1-RR

Synonyms

DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

Validation Data Gallery

Applications:
  • IHC staining of mouse spleen using 81668-1-RR

    IHC staining of mouse spleen using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IHC staining of mouse spleen using 81668-1-RR

    IHC staining of mouse spleen using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IHC staining of mouse liver using 81668-1-RR

    IHC staining of mouse liver using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IHC staining of mouse liver using 81668-1-RR

    IHC staining of mouse liver using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IF Staining of mouse spleen using 81668-1-RR

    IF Staining of mouse spleen using 81668-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse spleen using 81668-1-RR

    IF Staining of mouse spleen using 81668-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:10000
Immunofluorescence (IF)IF : 1:750-1:3000
Sample-dependent, check data in validation data gallery

Product Information

81668-1-RR targets F4/80 in IF, IHC, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen F4/80 fusion protein Ag28252 相同性解析による交差性が予測される生物種
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Calculated molecular weight 102 kDa
GenBank accession numberNM_010130
Gene symbol Emr1
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Protocols

Product Specific Protocols
IHC protocol for F4/80 antibody 81668-1-RRDownload protocol
IF protocol for F4/80 antibody 81668-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols