mouse F4/80 Polyclonal antibody
F4/80 Polyclonal Antibody for FC, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse, Rat and More (4)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
152
Cat no : 28463-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse spleen tissue, mouse liver tissue, RAW 264.7 cells |
| Positive FC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:4000-1:12000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 12 publications below |
| IHC | See 79 publications below |
| IF | See 59 publications below |
| FC | See 8 publications below |
Product Information
28463-1-AP targets F4/80 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Mouse, Rat samples.
| Tested Reactivity | Mouse, Rat |
| Cited Reactivity | human, rat, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | F4/80 fusion protein Ag28252 相同性解析による交差性が予測される生物種 |
| Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
| Calculated molecular weight | 102 kDa |
| GenBank accession number | NM_010130 |
| Gene symbol | Emr1 |
| Gene ID (NCBI) | 13733 |
| RRID | AB_2881149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for F4/80 antibody 28463-1-AP | Download protocol |
| IF protocol for F4/80 antibody 28463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia. | ||
Biomaterials A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis. | ||
Aging Dis P-Glycoprotein Aggravates Blood Brain Barrier Dysfunction in Experimental Ischemic Stroke by Inhibiting Endothelial Autophagy | ||
Autophagy Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. | ||
Acta Biomater An electrospun scaffold functionalized with a ROS-scavenging hydrogel stimulates ocular wound healing |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kannan (Verified Customer) (06-13-2023) | AR using EDTApH9. overnight incubation of primary antibody at 4c.
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