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- KD/KO Validated
EXOSC8 Polyclonal antibody
EXOSC8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11979-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue, human colon tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
11979-1-AP targets EXOSC8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EXOSC8 fusion protein Ag2605 相同性解析による交差性が予測される生物種 |
| Full Name | exosome component 8 |
| Calculated molecular weight | 276 aa, 30 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC020773 |
| Gene symbol | EXOSC8 |
| Gene ID (NCBI) | 11340 |
| RRID | AB_2101981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IHC protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IF protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IP protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.
| ||
Nat Commun EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.
| ||
PLoS One Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA. | ||
Neuromuscul Disord New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient. | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tsimafei (Verified Customer) (12-04-2022) | 50µg of total protein were used. Incubation ON at 4C
 |
FH Nikolaus (Verified Customer) (05-24-2022) | Antibodies applied after 2 rounds of stripping, signal still satisfactory
 |
