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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

SEC6 Polyclonal antibody

SEC6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14703-1-AP

Synonyms

EXOC3, exocyst complex component 3, Exocyst complex component Sec6, SEC6, SEC6L1, Sec6p

Validation Data Gallery

Applications:
  • WB analysis of human brain using 14703-1-AP

    WB analysis of human brain using 14703-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14703-1-AP

    WB analysis of HeLa using 14703-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 14703-1-AP

    WB analysis of COLO 320 using 14703-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 14703-1-AP

    WB analysis of HepG2 using 14703-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 14703-1-AP

    IHC staining of human gliomas using 14703-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 14703-1-AP

    IHC staining of human gliomas using 14703-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 14703-1-AP

    IHC staining of human gliomas using 14703-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 14703-1-AP

    IF Staining of HepG2 using 14703-1-AP

    Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14703-1-AP targets SEC6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC6 fusion protein Ag6383 相同性解析による交差性が予測される生物種
Full Name exocyst complex component 3
Calculated molecular weight 87 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC064569
Gene symbol EXOC3
Gene ID (NCBI) 11336
RRIDAB_2101542
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

Protocols

Product Specific Protocols
WB protocol for SEC6 antibody 14703-1-APDownload protocol
IHC protocol for SEC6 antibody 14703-1-APDownload protocol
IF protocol for SEC6 antibody 14703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Microbiol

Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells.

Authors - Hiroki Takeuchi
  • KD Validated
View Article
humanWB

Cell Microbiol

Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.

Authors - Takeuchi Hiroki H
  • KD Validated
View Article
ratWB

Dev Biol

Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition.

Authors - Youssef G G
View Article