Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 11109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11109-1-AP
WB result of ETFDH antibody (11109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETFDH transfected HEK-293 cells.
WB analysis of HEK-293 using 11109-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11109-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11109-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 11109-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11109-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11109-1-AP
IP result of anti-ETFDH (IP:11109-1-AP, 3ug; Detection:11109-1-AP 1:500) with mouse brain tissue lysate 8000ug.
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ETFDH Polyclonal antibody

ETFDH Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11109-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

EC:1.5.5.1, Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial, Electron-transferring-flavoprotein dehydrogenase, ETFQO, ETF-QO

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HEK-293 cells, rat brain tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue, human kidney tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 2 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

11109-1-AP targets ETFDH in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1568 Product name: Recombinant human ETFDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 269-617 aa of BC011890 Sequence: QTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM 相同性解析による交差性が予測される生物種
Full Name	electron-transferring-flavoprotein dehydrogenase
Calculated molecular weight	64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC011890
Gene Symbol	ETFDH
Gene ID (NCBI)	2110
RRID	AB_2231382
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16134
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) is also named as ETF-QO and belongs to the ETF-QO/fixC family.It is a 64-kDa monomer integrated in the inner mitochondrial membrane, con- tains one molecule of FAD and a 4Fe4S cluster(PMID:12815589).Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C).This antibody is speicific to ETFDH.

Protocols

Product Specific Protocols
IHC protocol for ETFDH antibody 11109-1-AP	Download protocol
IP protocol for ETFDH antibody 11109-1-AP	Download protocol
WB protocol for ETFDH antibody 11109-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cell Death Dis The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR Authors - Xiaoya Sun View Article
mouse	WB,CoIP	Int J Biol Sci Metformin Alleviates Liver Metabolic Dysfunction in Polycystic Ovary Syndrome by Activating the Ethe1/Keap1/PINK1 Pathway Authors - Yuan Xie View Article
		Cell Commun Signal Genotype-environment interaction drives the onset of riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency in carriers of single heterozygous ETFDH variants. Authors - Jing Ma View Article
		Adv Healthc Mater A Biomimetic Copper-Caffeic Acid Nanozyme Activates Cuproptosis and Pyroptosis by Mimicking the Neutrophil Enzymatic Cascade. Authors - Yangyang Lu View Article
human	WB	Free Radic Biol Med Mitochondrial uncoupling sensitizes gastric cancer cells to elesclomol-induced cuproptosis via FDX1/DLAT upregulation. Authors - Yangyang Lu View Article
human	WB	J Neurol Neurosurg Psychiatry Riboflavin-responsive lipid-storage myopathy caused by ETFDH gene mutations. Authors - Wen Bing B View Article

Validation Data Gallery

WB analysis using 11109-1-AP

WB analysis of HEK-293 using 11109-1-AP

WB analysis of HEK-293 using 11109-1-AP

WB analysis of rat brain using 11109-1-AP

WB analysis of mouse brain using 11109-1-AP

WB analysis of mouse kidney using 11109-1-AP

WB analysis using 11109-1-AP

WB analysis of mouse skeletal muscle using 11109-1-AP

IP experiment of mouse brain using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

IHC staining of human prostate cancer using 11109-1-AP

IHC staining of human prostate cancer using 11109-1-AP

IHC staining of human kidney using 11109-1-AP

IHC staining of human kidney using 11109-1-AP

IHC staining of human liver using 11109-1-AP

IHC staining of human liver using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

ETFDH Polyclonal antibody

ETFDH Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR

Metformin Alleviates Liver Metabolic Dysfunction in Polycystic Ovary Syndrome by Activating the Ethe1/Keap1/PINK1 Pathway

Genotype-environment interaction drives the onset of riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency in carriers of single heterozygous ETFDH variants.

A Biomimetic Copper-Caffeic Acid Nanozyme Activates Cuproptosis and Pyroptosis by Mimicking the Neutrophil Enzymatic Cascade.

Mitochondrial uncoupling sensitizes gastric cancer cells to elesclomol-induced cuproptosis via FDX1/DLAT upregulation.

Riboflavin-responsive lipid-storage myopathy caused by ETFDH gene mutations.

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