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EPHB4 Polyclonal antibody

EPHB4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20883-1-AP

Synonyms

EPH receptor B4, EPHB4, Ephrin type B receptor 4, HTK, MYK1, TYRO11, Tyrosine protein kinase TYRO11



Tested Applications

Positive WB detected inHT-29 cells, rat testis tissue, HeLa cells, HT-1080 cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

20883-1-AP targets EPHB4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPHB4 fusion protein Ag10033 相同性解析による交差性が予測される生物種
Full Name EPH receptor B4
Calculated molecular weight 987 aa, 108 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC052804
Gene symbol EPHB4
Gene ID (NCBI) 2050
RRIDAB_10897158
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EPHB4 antibody 20883-1-APDownload protocol
IHC protocol for EPHB4 antibody 20883-1-APDownload protocol
IF protocol for EPHB4 antibody 20883-1-APDownload protocol
FC protocol for EPHB4 antibody 20883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Small

Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.

Authors - Xiaomeng Guo
humanIF

Drug Deliv

Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.

Authors - Zuhua Wang
WB,IHC

J Cell Physiol

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.

Authors - Tiancheng Li
humanWB

J Cell Mol Med

Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.

Authors - Immacolata Andolfo
humanFC

Phytomedicine

Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.

Authors - Qi Su
ratIHC

Commun Biol

The application of tissue-engineered fish swim bladder vascular graft.

Authors - Hualong Bai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marsilius (Verified Customer) (04-13-2021)

This antibody is useful for staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: brain
EPHB4 Antibody Immunofluorescence validation (1:100 dilution) in brain (Cat no:20883-1-AP)