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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EMILIN1 Monoclonal antibody

EMILIN1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5C8

Cat no : 60047-1-Ig

Synonyms

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

Validation Data Gallery

Applications:
  • WB analysis of human colon using 60047-1-Ig

    WB analysis of human colon using 60047-1-Ig

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • WB analysis of human rectum using 60047-1-Ig

    WB analysis of human rectum using 60047-1-Ig

    human rectum tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human colon using 60047-1-Ig

    WB analysis of human colon using 60047-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 60047-1-Ig

    IHC staining of human kidney using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 60047-1-Ig

    IHC staining of human kidney using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 60047-1-Ig

    IHC staining of human colon using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 60047-1-Ig

    IHC staining of human colon using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 60047-1-Ig

    IHC staining of human ovary tumor using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 60047-1-Ig

    IHC staining of human ovary tumor using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 60047-1-Ig

    IHC staining of human ovary tumor using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 60047-1-Ig

    IHC staining of human ovary tumor using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 60047-1-Ig

    IHC staining of human lung cancer using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 60047-1-Ig

    IHC staining of human lung cancer using 60047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human ovary tumor using 60047-1-Ig

    IF Staining of human ovary tumor using 60047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human ovary tumor using 60047-1-Ig

    IF Staining of human ovary tumor using 60047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman colon tissue, human rectum tissue
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman ovary tumor tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60047-1-Ig targets EMILIN1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EMILIN1 fusion protein Ag1016 相同性解析による交差性が予測される生物種
Full Name elastin microfibril interfacer 1
Calculated molecular weight 107 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC007530
Gene symbol EMILIN1
Gene ID (NCBI) 11117
RRIDAB_2100191
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

Protocols

Product Specific Protocols
WB protocol for EMILIN1 antibody 60047-1-IgDownload protocol
IHC protocol for EMILIN1 antibody 60047-1-IgDownload protocol
IF protocol for EMILIN1 antibody 60047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Connect Tissue Res

EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro.

Authors - Nakatomi Yuka Y
View Article
humanIHC

Hernia

Elastic fibers in the anterior abdominal wall.

Authors - Fachinelli A A
View Article