Featured Product
KD/KO Validated

ELL Polyclonal antibody

Name: ELL Polyclonal antibody
Brand: Proteintech
SKU: 51044-1-AP

ELL Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 51044-1-AP

Synonyms

C19orf17, ELL, ELL1, MEN

Validation Data Gallery

Applications:

AllWBIHC

WB analysis of HeLa using 51044-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 51044-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 51044-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 51044-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 51044-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 51044-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51044-1-AP (ELL antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 51044-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

51044-1-AP targets ELL in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ELL fusion protein Ag0617 相同性解析による交差性が予測される生物種
Full Name	elongation factor RNA polymerase II
Calculated molecular weight	500 aa, 68 kDa
Observed molecular weight	68-80 kDa
GenBank accession number	BC049195
Gene symbol	ELL
Gene ID (NCBI)	8178
RRID	AB_2099137
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.

Protocols

Product Specific Protocols
WB protocol for ELL antibody 51044-1-AP	Download protocol
IHC protocol for ELL antibody 51044-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity. Authors - Chia-Yu Chung KD Validated View Article
human	WB	Nat Commun ELL targets c-Myc for proteasomal degradation and suppresses tumour growth. Authors - Yu Chen View Article
mouse	WB	Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. Authors - Hao Wang View Article
human	WB	Prostate ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells. Authors - Liu Lingqi L KD Validated View Article
human	WB	Mol Immunol Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival. Authors - Lou-Ella M M Alexander KD Validated View Article
mouse	WB,IP	Cell Inherited blood cancer predisposition through altered transcription elongation Authors - Jiawei Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ELL Polyclonal antibody

ELL Polyclonal Antibody for IHC, WB,ELISA

Validation Data Gallery

WB analysis of HeLa using 51044-1-AP

WB analysis of mouse testis using 51044-1-AP

WB analysis of HL-60 using 51044-1-AP

WB analysis of HEK-293 using 51044-1-AP

WB analysis of HeLa using 51044-1-AP

IHC staining of human ovary tumor using 51044-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.

ELL targets c-Myc for proteasomal degradation and suppresses tumour growth.

The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.

ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.

Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.

Inherited blood cancer predisposition through altered transcription elongation