Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, Neuro-2a cells |
| Positive IP detected in | Neuro-2a cells |
| Positive IHC detected in | human lung cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IF | See 4 publications below |
| RIP | See 1 publications below |
Product Information
55047-1-AP targets HuC in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 36-38 kDa |
| GenBank accession number | NM_001420 |
| Gene Symbol | HuC |
| Gene ID (NCBI) | 1995 |
| RRID | AB_10859256 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HuC antibody 55047-1-AP | Download protocol |
| IHC protocol for HuC antibody 55047-1-AP | Download protocol |
| IP protocol for HuC antibody 55047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. | ||
Invest Ophthalmol Vis Sci Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function | ||
Am J Pathol Anaplastic lymphoma kinase (ALK) overexpression is associated with aggressive phenotypic characteristics of ovarian high-grade serous carcinoma. | ||
Cell Stem Cell KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury | ||
iScience Multivalent GU-rich oligonucleotides sequester TDP-43 in the nucleus by inducing high molecular weight RNP complexes |