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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF5A2 Monoclonal antibody

EIF5A2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9B2

Cat no : 67907-1-Ig

Synonyms

eIF 5A 2, EIF 5A2, EIF5A2, eIF5AII

Validation Data Gallery

Applications:
  • WB analysis using 67907-1-Ig

    WB analysis using 67907-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67907-1-Ig (EIF5A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung cancer using 67907-1-Ig

    IHC staining of human lung cancer using 67907-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67907-1-Ig

    IHC staining of human lung cancer using 67907-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 67907-1-Ig

    IHC staining of human stomach cancer using 67907-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 67907-1-Ig

    IHC staining of human stomach cancer using 67907-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67907-1-Ig

    IF Staining of HepG2 using 67907-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF5A2 antibody (67907-1-Ig, Clone: 1F9B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67907-1-Ig targets EIF5A2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF5A2 fusion protein Ag10852 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 5A2
Calculated molecular weight 153 aa, 17 kDa
Observed molecular weight20 kDa
GenBank accession numberBC036072
Gene symbol EIF5A2
Gene ID (NCBI) 56648
RRIDAB_2918663
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.

Protocols

Product Specific Protocols
WB protocol for EIF5A2 antibody 67907-1-IgDownload protocol
IHC protocol for EIF5A2 antibody 67907-1-IgDownload protocol
IF protocol for EIF5A2 antibody 67907-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols