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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated EIF4G1 Monoclonal antibody

EIF4G1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10G8

Cat no : CL488-67199

Synonyms

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-67199

    IF Staining of HepG2 using CL488-67199

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 EIF4G1 antibody (CL488-67199, Clone: 2B10G8 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4G1 Ready-To-Use IHC Kit. EIF4G1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67199-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67199 targets EIF4G1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8357 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated molecular weight 1600 aa, 176 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC007788
Gene symbol EIF4G1
Gene ID (NCBI) 1981
RRIDAB_2919434
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EIF4G1 antibody CL488-67199Download protocol
Standard Protocols
Click here to view our Standard Protocols