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EIF3I Polyclonal antibody

Name: EIF3I Polyclonal antibody
Brand: Proteintech
SKU: 11287-1-AP
Rating: 5.00 (1 reviews)

EIF3I Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11287-1-AP

Synonyms

eIF 3 beta, eIF3 beta, eIF3 p36, EIF3I, EIF3S2, PRO2242, TRIP 1, TRIP1

Validation Data Gallery

Applications:

AllWBIPIHCIFFC

WB analysis of HEK-293 using 11287-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11287-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11287-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11287-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 11287-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11287-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11287-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11287-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11287-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11287-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11287-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 11287-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11287-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11287-1-AP (EIF3I antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11287-1-AP
IP result of anti-EIF3I (IP:11287-1-AP, 3ug; Detection:11287-1-AP 1:1000) with HEK-293 cells lysate 1500ug.
IHC staining of human breast cancer using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11287-1-AP (EIF3I antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human breast cancer using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11287-1-AP (EIF3I antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human liver using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human liver using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human testis using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human testis using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human breast cancer using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with EIF3I antibody included
IHC staining of human breast cancer using 11287-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11287-1-AP (EIF3I antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with EIF3I antibody included
IF Staining of HeLa using 11287-1-AP
Immunofluorescent analysis of Hela cells, using EIF3I antibody 11287-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HeLa using 11287-1-AP
1X10^6 HeLa cells were stained with 0.2ug EIF3I antibody (11287-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 11287-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells, human placenta tissue, NIH/3T3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human liver tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	Hela cells

Planning an IHC experiment? We recommend our IHCeasy EIF3I Ready-To-Use IHC Kit. EIF3I primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 9 publications below
IHC	See 2 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

11287-1-AP targets EIF3I in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF3I fusion protein Ag1847 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 3, subunit I
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC000413
Gene symbol	EIF3I
Gene ID (NCBI)	8668
RRID	AB_2097068
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.

Protocols

Product Specific Protocols
WB protocol for EIF3I antibody 11287-1-AP	Download protocol
IHC protocol for EIF3I antibody 11287-1-AP	Download protocol
IF protocol for EIF3I antibody 11287-1-AP	Download protocol
IP protocol for EIF3I antibody 11287-1-AP	Download protocol
FC protocol for EIF3I antibody 11287-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Metab Eng Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis. Authors - Anne Roobol View Article
rat	IHC,WB	J Bone Miner Res TRIP-1: a regulator of osteoblast function. Authors - Metz-Estrella Diana D KD Validated View Article
mouse, hamster	WB	Biochem J The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h. Authors - Anne Roobol View Article
human	WB,IF,CoIP	Ecotoxicol Environ Saf Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway. Authors - Yujing Zhang View Article
human	WB	Biochem J Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity. Authors - Emma Jane Mead View Article
human	WB	Vet Microbiol EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein. Authors - Wei Pan KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Fabienne (Verified Customer) (06-26-2023)

the antibody works well for Western Blot

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: HEK293

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3I Polyclonal antibody

EIF3I Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Validation Data Gallery

WB analysis of HEK-293 using 11287-1-AP

WB analysis of mouse brain using 11287-1-AP

WB analysis of MCF-7 using 11287-1-AP

WB analysis of K-562 using 11287-1-AP

WB analysis of SH-SY5Y using 11287-1-AP

WB analysis of human placenta using 11287-1-AP

WB analysis of HeLa using 11287-1-AP

WB analysis of HepG2 using 11287-1-AP

WB analysis of HEK-293 using 11287-1-AP

WB analysis of Jurkat using 11287-1-AP

WB analysis of NIH/3T3 using 11287-1-AP

WB analysis of SH-SY5Y using 11287-1-AP

WB analysis of Jurkat using 11287-1-AP

IP experiment of HEK-293 using 11287-1-AP

IHC staining of human breast cancer using 11287-1-AP

IHC staining of human breast cancer using 11287-1-AP

IHC staining of human liver using 11287-1-AP

IHC staining of human liver using 11287-1-AP

IHC staining of human testis using 11287-1-AP

IHC staining of human testis using 11287-1-AP

IHC staining of human breast cancer using 11287-1-AP

IHC staining of human breast cancer using 11287-1-AP

IF Staining of HeLa using 11287-1-AP

FC experiment of HeLa using 11287-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis.

TRIP-1: a regulator of osteoblast function.

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.

Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.

Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.

EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein.

Reviews