Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 26178-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 26178-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26178-1-AP
WB result of EIF3A antibody (26178-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF3A transfected A549 cells.
WB analysis using 26178-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 26178-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 26178-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF3A antibody (26178-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

EIF3A Polyclonal antibody

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26178-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, mouse brain tissue
Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IF	See 1 publications below
RIP	See 1 publications below

Product Information

26178-1-AP targets EIF3A in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22515 Product name: Recombinant human EIF3A protein Source: e coli.-derived, PKG Tag: GST Domain: 1033-1382 aa of BC114429 Sequence: DEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPGRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 3, subunit A
Calculated molecular weight	1382 aa, 166 kDa
Observed molecular weight	166 kDa
GenBank accession number	BC114429
Gene Symbol	EIF3A
Gene ID (NCBI)	8661
RRID	AB_2880413
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14152
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for EIF3A antibody 26178-1-AP	Download protocol
IHC protocol for EIF3A antibody 26178-1-AP	Download protocol
WB protocol for EIF3A antibody 26178-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Nucleic Acids Res PMID: 30295850 Authors - Zonghong Li View Article
human	WB,IF	Front Cell Infect Microbiol Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. Authors - Tian Luo View Article
human	WB	Onco Targets Ther WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling. Authors - Jingjing Dai View Article
mouse	WB	J Clin Lab Anal Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling. Authors - Zhenwei Li View Article
human	RIP	Proc Natl Acad Sci U S A Epitranscriptic regulation of HRAS by N6-methyladenosine drives tumor progression Authors - Yongbo Pan View Article
human	WB	iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges Authors - Tamami Miyagi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 26178-1-AP

WB analysis using 26178-1-AP

WB analysis of A549 using 26178-1-AP

WB analysis using 26178-1-AP

WB analysis of HEK-293 using 26178-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

IF Staining of HEK-293 using 26178-1-AP

EIF3A Polyclonal antibody

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 30295850

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Epitranscriptic regulation of HRAS by N6-methyladenosine drives tumor progression

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges