Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 18244-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 18244-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18244-1-AP
WB result of EIF2AK2, PKR antibody (18244-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK2, PKR transfected HepG2 cells.
WB analysis of rat heart using 18244-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18244-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 18244-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 18244-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 18244-1-AP
IP result of anti-EIF2AK2, PKR (IP:18244-1-AP, 4ug; Detection:18244-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of human kidney using 18244-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18244-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18244-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18244-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 18244-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 18244-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18244-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF2AK2, PKR antibody (18244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18244-1-AP

Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

EIF2AK2, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (53)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 45 publications below
IHC	See 3 publications below
IF	See 4 publications below
IP	See 2 publications below
RIP	See 2 publications below

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, rabbit, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13010 Product name: Recombinant human EIF2AK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC093676 Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 2-alpha kinase 2
Calculated molecular weight	551 aa, 62 kDa
Observed molecular weight	65-68 kDa
GenBank accession number	BC093676
Gene Symbol	EIF2AK2
Gene ID (NCBI)	5610
RRID	AB_2246451
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19525
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for EIF2AK2, PKR antibody 18244-1-AP	Download protocol
IHC protocol for EIF2AK2, PKR antibody 18244-1-AP	Download protocol
IP protocol for EIF2AK2, PKR antibody 18244-1-AP	Download protocol
WB protocol for EIF2AK2, PKR antibody 18244-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. Authors - Alessandro Carugo View Article
mouse	WB	Nat Neurosci Maternal immune activation in mice disrupts proteostasis in the fetal brain. Authors - Brian T Kalish View Article
human	WB	Sci Adv Temporal control of the integrated stress response by a stochastic molecular switch. Authors - Philipp Klein View Article
human	WB	Nat Commun NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice. Authors - Psylvia Léger View Article
human	WB	J Immunother Cancer Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment Authors - Weiwei Lin View Article
human,mouse	WB	Int J Antimicrob Agents Pan-viral propagation blockade by inhibiting host cell PNPT1 Authors - Shuang Qu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18244-1-AP

WB analysis using 18244-1-AP

WB analysis of HepG2 using 18244-1-AP

WB analysis of rat heart using 18244-1-AP

WB analysis of HeLa using 18244-1-AP

WB analysis of mouse thymus using 18244-1-AP

WB analysis using 18244-1-AP

IP experiment of HeLa using 18244-1-AP

IHC staining of human kidney using 18244-1-AP

IHC staining of human kidney using 18244-1-AP

IHC staining of human kidney using 18244-1-AP

IHC staining of human kidney using 18244-1-AP

IHC staining of human testis using 18244-1-AP

IHC staining of human testis using 18244-1-AP

IF Staining of HeLa using 18244-1-AP

EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Maternal immune activation in mice disrupts proteostasis in the fetal brain.

Temporal control of the integrated stress response by a stochastic molecular switch.

NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.

Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment

Pan-viral propagation blockade by inhibiting host cell PNPT1