Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, Jurkat cells, HEK-293 cells |
| Positive IP detected in | PC-3 cells |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13510-1-AP targets eEF2K in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4432 Product name: Recombinant human EEF2K protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 376-725 aa of BC032665 Sequence: LSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic elongation factor-2 kinase |
| Calculated molecular weight | 725 aa, 100 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC032665 |
| Gene Symbol | EEF2K |
| Gene ID (NCBI) | 29904 |
| RRID | AB_2277761 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00418 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for eEF2K antibody 13510-1-AP | Download protocol |
| IP protocol for eEF2K antibody 13510-1-AP | Download protocol |
| WB protocol for eEF2K antibody 13510-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells. | ||
Oncol Lett AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K. | ||
Cells SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.
| ||
Med Oncol A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells | ||
JOR Spine Molecular Subtypes and Immune Microenvironment Characterization of the Annulus Fibrosus in Intervertebral Disc Degeneration: Insights From Translation Factor-Related Gene Analysis | ||
J Mol Med (Berl) FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells. |
