Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, A431 cells, MCF-7 cells |
| Positive IP detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
18032-1-AP targets EDAR in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12601 Product name: Recombinant human EDAR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-448 aa of BC093872 Sequence: MFYILKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKDEFEKLTATPAKPTKSENDASSENEQLLSRSVDSDEEPAPDKQGSPELCLLSLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPFDCLEKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRISTAGYSIPELLTKLVQIERLDAVESLCADILEWAGVVPPASQPHAAS 相同性解析による交差性が予測される生物種 |
| Full Name | ectodysplasin A receptor |
| Calculated molecular weight | 448 aa, 49 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC093872 |
| Gene Symbol | EDAR |
| Gene ID (NCBI) | 10913 |
| RRID | AB_10596932 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNE0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for EDAR antibody 18032-1-AP | Download protocol |
| IP protocol for EDAR antibody 18032-1-AP | Download protocol |
| WB protocol for EDAR antibody 18032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Development Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. | ||
J Pharm Pharmacol Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway. | ||
J Histochem Cytochem Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice | ||
J Invest Dermatol ASH2L mediates epidermal differentiation and hair follicle morphogenesis via H3K4me3 modification |
