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  • Featured Product
  • KD/KO Validated

EBI3 Polyclonal antibody

EBI3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12371-1-AP

Synonyms

EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta

Validation Data Gallery

Applications:
  • WB analysis using 12371-1-AP

    WB analysis using 12371-1-AP

    human placenta tissue was subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12371-1-AP

    WB analysis using 12371-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 12371-1-AP

    IP experiment of human placenta using 12371-1-AP

    IP result of anti-EBI3 (IP:12371-1-AP, 4ug; Detection:12371-1-AP 1:800) with human placenta tissue lysate 600 ug.

  • IHC staining of human spleen using 12371-1-AP

    IHC staining of human spleen using 12371-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 12371-1-AP

    IHC staining of human spleen using 12371-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 12371-1-AP

    IHC staining of human placenta using 12371-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 12371-1-AP

    IHC staining of human placenta using 12371-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue, pig spleen tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12371-1-AP targets EBI3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EBI3 fusion protein Ag3051 相同性解析による交差性が予測される生物種
Full Name Epstein-Barr virus induced 3
Calculated molecular weight 229 aa, 25 kDa
Observed molecular weight 25-35 kDa
GenBank accession numberBC015364
Gene symbol EBI3
Gene ID (NCBI) 10148
RRIDAB_10646470
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)

Protocols

Product Specific Protocols
WB protocol for EBI3 antibody 12371-1-APDownload protocol
IHC protocol for EBI3 antibody 12371-1-APDownload protocol
IP protocol for EBI3 antibody 12371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Immunol

Role of IL-6-IL-27 Complex in Host Antiviral Immune Response.

Authors - Qi Zuo
View Article
humanWB,IF

PLoS One

Epstein-Barr virus-induced gene 3 commits human mesenchymal stem cells to differentiate into chondrocytes via endoplasmic reticulum stress sensor

Authors - Tong Zhang
View Article
mouseIF

Pathog Dis

Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.

Authors - Jia-Hui Deng
  • KO Validated
View Article