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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E-selectin / CD62E Polyclonal antibody

E-selectin / CD62E Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30205-1-AP

Synonyms

CD62E, E selectin, ELAM, ELAM 1, ELAM1, ESEL, LECAM2, SELE, selectin E

Validation Data Gallery

Applications:
  • IHC staining of human appendicitis using 30205-1-AP

    IHC staining of human appendicitis using 30205-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 30205-1-AP

    IHC staining of human appendicitis using 30205-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using 30205-1-AP

    IF Staining of human appendicitis using 30205-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human appendicitis using 30205-1-AP

    IF Staining of human appendicitis using 30205-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman appendicitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30205-1-AP targets E-selectin / CD62E in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-selectin / CD62E fusion protein Ag31820 相同性解析による交差性が予測される生物種
Full Name selectin E
Calculated molecular weight 610 aa, 67 kDa
GenBank accession numberBC142711
Gene symbol SELE
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

Protocols

Product Specific Protocols
IHC protocol for E-selectin / CD62E antibody 30205-1-APDownload protocol
IF protocol for E-selectin / CD62E antibody 30205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols