Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 cells using 12037-1-AP
WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 and si-control transfected cells.
WB analysis using 12037-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12037-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12037-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of MDA-MB-453s using 12037-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 12037-1-AP
IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:4000) with MCF-7 cells lysate 640 ug.
IHC staining of human lung cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12037-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DVL2 antibody (12037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

DVL2 Polyclonal antibody

DVL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12037-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Dishevelled 2, Segment polarity protein dishevelled homolog DVL-2, DSH homolog 2, Dishevelled-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse thymus tissue, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, MDA-MB-231 cells, NIH/3T3 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 29 publications below
IHC	See 4 publications below
IF	See 7 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

12037-1-AP targets DVL2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2666 Product name: Recombinant human DVL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC014844 Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI 相同性解析による交差性が予測される生物種
Full Name	dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight	736 aa, 79 kDa
Observed molecular weight	90-95 kDa
GenBank accession number	BC014844
Gene Symbol	DVL2
Gene ID (NCBI)	1856
RRID	AB_2093330
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14641
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.

Protocols

Product Specific Protocols
IF protocol for DVL2 antibody 12037-1-AP	Download protocol
IHC protocol for DVL2 antibody 12037-1-AP	Download protocol
IP protocol for DVL2 antibody 12037-1-AP	Download protocol
WB protocol for DVL2 antibody 12037-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Cell Biol Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling. Authors - Andre Landin Malt View Article
mouse	WB	Biol Res Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model Authors - Yanyi Ding View Article
human	WB	mBio Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner Authors - Xia-Ming Jiang View Article
human	WB	Cell Death Dis YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury. Authors - Feihong Deng View Article
human	WB	Development The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning. Authors - Verena Rauschenberger View Article
mouse	IHC	Front Cell Dev Biol Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition. Authors - Andre Landin Malt View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 cells using 12037-1-AP

WB analysis using 12037-1-AP

WB analysis of mouse thymus using 12037-1-AP

WB analysis of MCF-7 using 12037-1-AP

WB analysis of MDA-MB-453s using 12037-1-AP

IP experiment of MCF-7 using 12037-1-AP

IHC staining of human lung cancer using 12037-1-AP

IHC staining of human lung cancer using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IHC staining of human prostate cancer using 12037-1-AP

IHC staining of human prostate cancer using 12037-1-AP

IHC staining of mouse colon using 12037-1-AP

IHC staining of mouse colon using 12037-1-AP

IHC staining of rat colon using 12037-1-AP

IHC staining of rat colon using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IF Staining of HepG2 using 12037-1-AP

DVL2 Polyclonal antibody

DVL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.

Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model

Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner

YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition.