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  • Featured Product
  • KD/KO Validated

DVL2 Polyclonal antibody

DVL2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12037-1-AP

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

Validation Data Gallery

Applications:
  • WB analysis of HepG2 cells using 12037-1-AP

    WB analysis of HepG2 cells using 12037-1-AP

    WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 and si-control transfected cells.

  • WB analysis using 12037-1-AP

    WB analysis using 12037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 12037-1-AP

    WB analysis of mouse thymus using 12037-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12037-1-AP

    WB analysis of MCF-7 using 12037-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • WB analysis of MDA-MB-453s using 12037-1-AP

    WB analysis of MDA-MB-453s using 12037-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 12037-1-AP

    IP experiment of MCF-7 using 12037-1-AP

    IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:4000) with MCF-7 cells lysate 640 ug.

  • IHC staining of human lung cancer using 12037-1-AP

    IHC staining of human lung cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human lung cancer using 12037-1-AP

    IHC staining of human lung cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human gliomas using 12037-1-AP

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human gliomas using 12037-1-AP

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human gliomas using 12037-1-AP

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human gliomas using 12037-1-AP

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human prostate cancer using 12037-1-AP

    IHC staining of human prostate cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human prostate cancer using 12037-1-AP

    IHC staining of human prostate cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of mouse colon using 12037-1-AP

    IHC staining of mouse colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of mouse colon using 12037-1-AP

    IHC staining of mouse colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of rat colon using 12037-1-AP

    IHC staining of rat colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of rat colon using 12037-1-AP

    IHC staining of rat colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IF Staining of HCT 116 using 12037-1-AP

    IF Staining of HCT 116 using 12037-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using DVL2 antibody (12037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 12037-1-AP

    IF Staining of HepG2 using 12037-1-AP

    Immunofluorescent analysis of HepG2 cells using 12037-1-AP (DVL2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells, mouse thymus tissue, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, MDA-MB-231 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHCT 116 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DVL2 Ready-To-Use IHC Kit. DVL2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

12037-1-AP targets DVL2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, pig, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DVL2 fusion protein Ag2666 相同性解析による交差性が予測される生物種
Full Name dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight 736 aa, 79 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC014844
Gene symbol DVL2
Gene ID (NCBI) 1856
RRIDAB_2093330
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.

Protocols

Product Specific Protocols
WB protocol for DVL2 antibody 12037-1-APDownload protocol
IHC protocol for DVL2 antibody 12037-1-APDownload protocol
IF protocol for DVL2 antibody 12037-1-APDownload protocol
IP protocol for DVL2 antibody 12037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.

Authors - Andre Landin Malt
View Article
humanWB

Cell Death Dis

YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.

Authors - Feihong Deng
View Article
humanWB

Development

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Authors - Verena Rauschenberger
View Article
mouseIHC

Front Cell Dev Biol

Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition.

Authors - Andre Landin Malt
View Article
humanWB,IF

FASEB J

Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible role in preeclampsia.

Authors - Manni Sun
View Article
humanWB, IP

Mol Biol Cell

Distinct functionality of Dishevelled isoforms on Ca2+/Calmodulin dependent protein kinase 2 (CamKII) in Xenopus gastrulation.

Authors - Marc Gentzel
View Article