Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human liver tissue, human kidney tissue, HEK-293 cells, Ramos cells, A549 cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13740-1-AP targets DUT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4677 Product name: Recombinant human DUT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC033645 Sequence: MPCSEETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN 相同性解析による交差性が予測される生物種 |
| Full Name | deoxyuridine triphosphatase |
| Calculated molecular weight | 252 aa, 27 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC033645 |
| Gene Symbol | DUT |
| Gene ID (NCBI) | 1854 |
| RRID | AB_2093170 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33316 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DUT antibody 13740-1-AP | Download protocol |
| IHC protocol for DUT antibody 13740-1-AP | Download protocol |
| IP protocol for DUT antibody 13740-1-AP | Download protocol |
| WB protocol for DUT antibody 13740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. | ||
Cell Chem Biol CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity. | ||
Bioconjug Chem ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase. | ||
Biochem Genet Identification of a Novel Mitochondrial-Related Gene Signature for BMSCs in Osteoporosis Combining Single-Cell and Bulk Transcriptome Data |
