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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DUSP11 Polyclonal antibody

DUSP11 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10204

Synonyms

DUSP11, PIR1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-10204

    IF Staining of HepG2 using CL488-10204

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DUSP11 antibody (CL488-10204) at dilution of 1:200.

  • IF Staining of A431 using CL488-10204

    IF Staining of A431 using CL488-10204

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DUSP11 antibody (CL488-10204) at dilution of 1:600.

Tested Applications

Positive IF detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

CL488-10204 targets DUSP11 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP11 fusion protein Ag0276 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 11 (RNA/RNP complex 1-interacting)
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC000346
Gene symbol DUSP11
Gene ID (NCBI) 8446
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DUSP11 antibody CL488-10204Download protocol
Standard Protocols
Click here to view our Standard Protocols