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- KD/KO Validated
DOM3Z Polyclonal antibody
DOM3Z Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 11015-2-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | mouse testis tissue, SH-SY5Y cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
11015-2-AP targets DOM3Z in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DOM3Z fusion protein Ag1491 相同性解析による交差性が予測される生物種 |
| Full Name | dom-3 homolog Z (C. elegans) |
| Calculated molecular weight | 43.6 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC009344 |
| Gene symbol | DOM3Z |
| Gene ID (NCBI) | 1797 |
| RRID | AB_2095257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| IHC protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| IP protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell 5' End Nicotinamide Adenine Dinucleotide Cap in Human Cells Promotes RNA Decay through DXO-Mediated deNADding.
| ||
Mol Cell A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.
| ||
RNA Unraveling regulation and new components of human P-bodies through a protein interaction framework and experimental validation. | ||
PLoS One 2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO. | ||
Nat Chem Biol Structural and mechanistic basis of mammalian Nudt12 RNA deNADding.
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