Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 13101-1-AP
WB result of DNAJC10 antibody (13101-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC10 transfected HeLa cells.
WB analysis using 13101-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13101-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13101-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13101-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13101-1-AP
IP result of anti-DNAJC10 (IP:13101-1-AP, 4ug; Detection:13101-1-AP 1:500) with HeLa cells lysate 1600 ug.
IHC staining of human pancreas cancer using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 13101-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

DNAJC10 Polyclonal antibody

DNAJC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13101-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:1.8.4.-, Endoplasmic reticulum DNA J domain-containing protein 5, ER resident protein ERdj5, ERdj5, ER-resident protein ERdj5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human stomach tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

13101-1-AP targets DNAJC10 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3737 Product name: Recombinant human DNAJC10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 690-793 aa of BC034713 Sequence: HWVIDFYAPWCGPCQNFAPEFELLARMIKGKVKAGKVDCQAYAQTCQKAGIRAYPTVKFYFYERAKRNFQEEQINTRDAKAIAALISEKLETLRNQGKRNKDEL 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily C, member 10
Calculated molecular weight	793 aa, 91 kDa
Observed molecular weight	80-90 kDa
GenBank accession number	BC034713
Gene Symbol	DNAJC10
Gene ID (NCBI)	54431
RRID	AB_10638440
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IXB1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNAJC10(DnaJ homolog subfamily C member 10)is also named as ERDJ5. The human ER-resident protein (ERdj5) ubiquitously expresses and is abundant in secretory tissues such as pancreas and testis and it may be involved in assisting protein folding and quality control in the ER(PMID:12411443). The deduced 793-amino acid protein has a calculated molecular mass of about 91 kD. ERDJ5 contains an N-terminal hydrophobic sequence, followed by a type III DnaJ domain, 4 thioredoxin-like domains, and a C-terminal KDEL endoplasmic reticulum (ER) retention signal. It can be modified by N-linked glycosylation(PMID:12446677). The ERdj5 antibody displays a higher affinity for endogenous ERdj5u.

Protocols

Product Specific Protocols
IF protocol for DNAJC10 antibody 13101-1-AP	Download protocol
IHC protocol for DNAJC10 antibody 13101-1-AP	Download protocol
IP protocol for DNAJC10 antibody 13101-1-AP	Download protocol
WB protocol for DNAJC10 antibody 13101-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB	Nat Cell Biol SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis. Authors - Shanshan Liu View Article
human	WB,IHC	Mol Biomed Endoplasmic reticulum-resident protein DNAJC10 inhibits glioblastoma metastasis by suppressing XBP-1s-driven EGFR transcription. Authors - Erdi Zhao KD Validated View Article
human	WB	Diabetes Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding. Authors - Duc T Tran View Article
		Haematologica DNAJC10 maintains survival and self-renewal of leukemia stem cells through PERK branch of the unfolded protein response Authors - Minjing Li View Article
human	WB,CoIP	Mol Cell Proteomics Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis Authors - Madison T. Wright View Article
human	WB,IF	Int Endod J Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA. Authors - Diamanti E E View Article

Validation Data Gallery

WB analysis of HeLa using 13101-1-AP

WB analysis using 13101-1-AP

WB analysis of HeLa using 13101-1-AP

WB analysis of HepG2 using 13101-1-AP

WB analysis of mouse liver using 13101-1-AP

IP experiment of HeLa using 13101-1-AP

IHC staining of human pancreas cancer using 13101-1-AP

IHC staining of human pancreas cancer using 13101-1-AP

IHC staining of human pancreas cancer using 13101-1-AP

IHC staining of human pancreas cancer using 13101-1-AP

IHC staining of human stomach using 13101-1-AP

IHC staining of human stomach using 13101-1-AP

IHC staining of human testis using 13101-1-AP

IHC staining of human testis using 13101-1-AP

IF Staining of HeLa using 13101-1-AP

DNAJC10 Polyclonal antibody

DNAJC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis.

Endoplasmic reticulum-resident protein DNAJC10 inhibits glioblastoma metastasis by suppressing XBP-1s-driven EGFR transcription.

Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.

DNAJC10 maintains survival and self-renewal of leukemia stem cells through PERK branch of the unfolded protein response

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis

Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA.

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