Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse testis using 17177-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 17177-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 17177-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 17177-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 17177-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 17177-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17177-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17177-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17177-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 17177-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

DNAJB3 Polyclonal antibody

DNAJB3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17177-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DNAJB3, HCG3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, rat testis tissue
Positive IHC detected in	mouse testis tissue, human kidney tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 2 publications below

Product Information

17177-1-AP targets DNAJB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10878 Product name: Recombinant human DNAJB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC024013 Sequence: MVDYYEVLDVPRQASSEAIKKAYRKLALKWHPDKNPENKEEAERRFKQVAEAYEVLSDAKKRDIYDRYGEAGAEGGCTGGRPFEDPFEYVFSFRDPADVFREFFGGQDPFSFDLLGNPLENILGGSEELLGKQKQSVCTPFLCLQ 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily B, member 3
Calculated molecular weight	145 aa, 17 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC024013
Gene Symbol	DNAJB3
Gene ID (NCBI)	414061
RRID	AB_2094420
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WWF6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for DNAJB3 antibody 17177-1-AP	Download protocol
WB protocol for DNAJB3 antibody 17177-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Rep PMID: 26400768 Authors - Mohamed Abu-Farha View Article
mouse	WB	Sci Rep Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone. Authors - Abdoulaye Diane KD Validated View Article
human	WB,IHC	PLoS One DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise. Authors - Abubaker Jehad J View Article
mouse	WB	Int J Mol Sci Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice. Authors - Ryo Mitsugi View Article
human	WB,IHC	Lipids Health Dis Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue. Authors - Ali Tiss View Article
mouse	WB	Sci Rep DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation. Authors - Abdelilah Arredouani KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 17177-1-AP

WB analysis using 17177-1-AP

WB analysis using 17177-1-AP

WB analysis of rat testis using 17177-1-AP

IHC staining of mouse testis using 17177-1-AP

IHC staining of mouse testis using 17177-1-AP

IHC staining of human kidney using 17177-1-AP

IHC staining of human kidney using 17177-1-AP

IHC staining of human ovary using 17177-1-AP

IHC staining of human ovary using 17177-1-AP

DNAJB3 Polyclonal antibody

DNAJB3 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 26400768

Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.

DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise.

Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice.

Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.

DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.