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  • Featured Product
  • KD/KO Validated

Biotin-conjugated DNAJB12 Polyclonal antibody

DNAJB12 Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-16780

Synonyms

DJ10, DKFZp586B2023, DNAJB12

Validation Data Gallery

Applications:
  • IHC staining of human colon cancer using Biotin-16780

    IHC staining of human colon cancer using Biotin-16780

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using Biotin-16780

    IHC staining of human colon cancer using Biotin-16780

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

Biotin-16780 targets DNAJB12 in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB12 fusion protein Ag10193 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 12
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC011812
Gene symbol DNAJB12
Gene ID (NCBI) 54788
Conjugate Biotin
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Biotin DNAJB12 antibody Biotin-16780Download protocol
Standard Protocols
Click here to view our Standard Protocols