Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse lung tissue, mouse ovary tissue |
| Positive IP detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9242 Product name: Recombinant human DMKN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-476 aa of BC011886 Sequence: SETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQPGAGWQEVAAVTSKNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASRVQPGLLQWVKFW 相同性解析による交差性が予測される生物種 |
| Full Name | dermokine |
| Calculated molecular weight | 476 aa, 47 kDa |
| Observed molecular weight | 48-55 kDa |
| GenBank accession number | BC011886 |
| Gene Symbol | DMKN |
| Gene ID (NCBI) | 93099 |
| RRID | AB_10596473 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6E0U4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for DMKN antibody 16252-1-AP | Download protocol |
| WB protocol for DMKN antibody 16252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome. | ||
Mol Cell Proteomics Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions. |
