Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human cervix tissue, human skin cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
21051-1-AP targets DKK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12405 Product name: Recombinant human DKK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC075078 Sequence: MAALMRSKDSSCCLLLLAAVLMVESSQIGSSRAKLNSIKSSLGGETPGQAANRSAGMYQGLAFGGSKKGKNLGQAYPCSSDKECEVGRYCHSPHQGSSACMVCRRKKKRCHRDGMCCPSTRCNNGICIPVTESILTPHIPALDGTRHRDRNHGHYSNHDLGWQNLGRPHTKMSHIKGHEGDPCLRSSDCIEGFCCARHFWTKICKPVLHQGEVCTKQRKKGSHGLEIFQRCDCAKGLSCKVWKDATYSSKARLHVCQKI 相同性解析による交差性が予測される生物種 |
| Full Name | dickkopf homolog 2 (Xenopus laevis) |
| Calculated molecular weight | 259 aa, 28 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC075078 |
| Gene Symbol | DKK2 |
| Gene ID (NCBI) | 27123 |
| RRID | AB_11232231 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DKK2 antibody 21051-1-AP | Download protocol |
| IP protocol for DKK2 antibody 21051-1-AP | Download protocol |
| WB protocol for DKK2 antibody 21051-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Neurobiol Ionizing Radiation Blocks Hair Cell Regeneration in Zebrafish Lateral Line Neuromasts by Preventing Wnt Signaling. | ||
Int J Mol Sci Roles of Dkk2 in the Linkage from Muscle to Bone during Mechanical Unloading in Mice. | ||
Front Endocrinol (Lausanne) G Protein-Coupled Estrogen Receptor Correlates With Dkk2 Expression and Has Prognostic Impact in Ovarian Cancer Patients. | ||
Exp Clin Endocrinol Diabetes Role of Dkk2 in the Muscle/bone Interaction of Androgen-Deficient Mice | ||
Cell Death Discov Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow. | ||
J Immunol Res DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma.
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