Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse ovary tissue, mouse testis tissue, HeLa cells, Jurkat cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14689-1-AP targets DIS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6117 Product name: Recombinant human DIS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 607-958 aa of BC056143 Sequence: TTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK 相同性解析による交差性が予測される生物種 |
| Full Name | DIS3 mitotic control homolog (S. cerevisiae) |
| Calculated molecular weight | 109 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC056143 |
| Gene Symbol | DIS3 |
| Gene ID (NCBI) | 22894 |
| RRID | AB_2091025 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2L1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DIS3 antibody 14689-1-AP | Download protocol |
| IHC protocol for DIS3 antibody 14689-1-AP | Download protocol |
| WB protocol for DIS3 antibody 14689-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs. | ||
Sci Adv Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection | ||
Nucleic Acids Res RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis. | ||
Proc Natl Acad Sci U S A The early macrophage response to pathogens requires dynamic regulation of the nuclear paraspeckle
| ||
EMBO J The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.
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