Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HEK-293 using 11355-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11355-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 11355-1-AP
IP result of anti-DHX58/LGP2 (IP:11355-1-AP, 3ug; Detection:11355-1-AP 1:300) with mouse liver tissue lysate 6000ug.
IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human nephroblastoma using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human nephroblastoma using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:10 (under 10x lens).

Featured Product
KD/KO Validated

DHX58/LGP2 Polyclonal antibody

DHX58/LGP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11355-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Synonyms

DHX58, LGP2, ATP-dependent helicase LGP2, ATP-dependent RNA helicase DHX58, D11LGP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, rat kidney tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue, human nephroblastoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 15 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

11355-1-AP targets DHX58/LGP2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, monkey, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1910 Product name: Recombinant human DHX58 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 330-678 aa of BC014949 Sequence: LFDDRKNELAHLATHGPENPKLEMLEKILQRQFSSSNSPRGIIFTRTRQSAHSLLLWLQQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQQEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSRELKRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKLPVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD 相同性解析による交差性が予測される生物種
Full Name	DEXH (Asp-Glu-X-His) box polypeptide 58
Calculated molecular weight	678 aa, 77 kDa
Observed molecular weight	77 kDa
GenBank accession number	BC014949
Gene Symbol	DHX58
Gene ID (NCBI)	79132
RRID	AB_2092319
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96C10
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DHX58(Probable ATP-dependent RNA helicase DHX58) is also named as D11LGP2E, LGP2 and belongs to the RLR subfamily. DHX58, originally identified as a highly expressed gene in mammary tumors, is another cytoplasmic DEX(D/H)-box helicase that can recognize RNA(PMID: 18411269). It acts as a positive, but not negative, regulator of RIG-I-and MDA5-dependent recognition of RNA virus infection and plays a pivotal role in antiviral responses in vivo(PMID:20080593).

Protocols

Product Specific Protocols
IHC protocol for DHX58/LGP2 antibody 11355-1-AP	Download protocol
IP protocol for DHX58/LGP2 antibody 11355-1-AP	Download protocol
WB protocol for DHX58/LGP2 antibody 11355-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection. Authors - Amy E L Stone KO Validated View Article
human	WB	Nucleic Acids Res miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11. Authors - Yalena Amador-Cañizares View Article
human	WB	EMBO Rep RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling. Authors - Jean-Patrick Parisien View Article
pig,monkey,human	WB,IP,IF	J Virol LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling Authors - Zhenbang Zhu KD Validated View Article
mouse,human	WB	J Virol Ambivalent role of the innate immune response in rabies virus pathogenesis. Authors - Chopy Damien D View Article
mouse,human	WB,IP,IHC	Cancer Sci Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma. Authors - Li-Ling Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 11355-1-AP

WB analysis using 11355-1-AP

IP experiment of mouse liver using 11355-1-AP

IHC staining of human breast cancer using 11355-1-AP

IHC staining of human breast cancer using 11355-1-AP

IHC staining of human nephroblastoma using 11355-1-AP

IHC staining of human nephroblastoma using 11355-1-AP

IHC staining of human breast cancer using 11355-1-AP

IHC staining of human breast cancer using 11355-1-AP

IHC staining of human breast cancer using 11355-1-AP

DHX58/LGP2 Polyclonal antibody

DHX58/LGP2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.

miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.

RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.