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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHFR Monoclonal antibody

DHFR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C11E9

Cat no : 68274-1-Ig

Synonyms

DHFR, DHFRP1, dihydrofolate reductase

Validation Data Gallery

Applications:
  • WB analysis using 68274-1-Ig

    WB analysis using 68274-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of NIH/3T3 using 68274-1-Ig

    WB analysis of NIH/3T3 using 68274-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

68274-1-Ig targets DHFR in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHFR fusion protein Ag7318 相同性解析による交差性が予測される生物種
Full Name dihydrofolate reductase
Calculated molecular weight 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC000192
Gene symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

Protocols

Product Specific Protocols
WB protocol for DHFR antibody 68274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols