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  • KD/KO Validated

DGKH Polyclonal antibody

DGKH Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13873-1-AP

Synonyms

DAG kinase eta, DGK eta, DGKeta, DGKH, Diacylglycerol kinase eta, diacylglycerol kinase, eta, Diglyceride kinase eta



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13873-1-AP targets DGKH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKH fusion protein Ag4926 相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, eta
Calculated molecular weight 1100 aa, 122 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC043292
Gene symbol DGKH
Gene ID (NCBI) 160851
RRIDAB_2091287
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGKH(Diacylglycerol kinase eta) is also named as DGK-eta and belongs to the eukaryotic diacylglycerol kinase family. It is a key protein in the lithium-sensitive phosphatidyl inositol pathway, and recently a link between environmental stress and schizophrenia/bipolar disorder has been revealed. DGKH has some isoforms produced by alternative splicing. Two isoforms DGKH1 and DGKH2 with the molecular weight of 128 kDa and 135 kDa shows diacylglycerol kinase activity; however, the 2 isoforms differed in several other characteristics(PMID:12810723).

Protocols

Product Specific Protocols
WB protocol for DGKH antibody 13873-1-APDownload protocol
IHC protocol for DGKH antibody 13873-1-APDownload protocol
IF protocol for DGKH antibody 13873-1-APDownload protocol
IP protocol for DGKH antibody 13873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

J Invest Dermatol

Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.

Authors - Kawaguchi Masakazu M
mouseWB

BMC Dev Biol

Distinct expression and localization of the type II diacylglycerol kinase isozymes ¿, ¿ and ¿ in the mouse reproductive organs.

Authors - Takao Shionoya
mouseWB

J Histochem Cytochem

Expression and Localization of Type II Diacylglycerol Kinase Isozymes δ and η in the Developing Mouse Brain.

Authors - Takako Usuki
human, mouseWB,IHC

Clin Transl Oncol

Diacylglycerol kinase η modulates oncogenic properties of lung cancer cells.

Authors - Nakano T T
  • KD Validated
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells.

Authors - Kai Iwata
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun