Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
25833-1-AP targets DEPP in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DEPP fusion protein Ag22773 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 10 open reading frame 10 |
| Calculated molecular weight | 212 aa, 23 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC011402 |
| Gene Symbol | DEPP |
| Gene ID (NCBI) | 11067 |
| RRID | AB_2880260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NTK1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DEPP, also named as FIG and C10orf10, is known to be differentially regulated in mouse tissues in response to hypoxia and oxidative stress. Moreover, Studies have reported that DEPP1 is one of the early adipogenic markers (PMID:36918557).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DEPP antibody 25833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma. | ||
Cell Death Dis Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.
| ||
Int J Oncol Baicalein induces the apoptosis of HCT116 human colon cancer cells via the upregulation of DEPP/Gadd45a and activation of MAPKs. | ||