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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2D3D2

Cat no : 67025-1-Ig

Synonyms

DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

Validation Data Gallery

Applications:
  • WB analysis using 67025-1-Ig

    WB analysis using 67025-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 67025-1-Ig

    WB analysis using 67025-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67025-1-Ig

    WB analysis using 67025-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 67025-1-Ig

    IP experiment of HeLa using 67025-1-Ig

    IP result of anti-DDX5 (IP:67025-1-Ig, 5ug; Detection:67025-1-Ig 1:1000) with HeLa cells lysate 1600 ug.

  • IHC staining of human colon cancer using 67025-1-Ig

    IHC staining of human colon cancer using 67025-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX5 antibody included
  • IHC staining of human colon cancer using 67025-1-Ig

    IHC staining of human colon cancer using 67025-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX5 antibody included
  • IF Staining of HepG2 using 67025-1-Ig

    IF Staining of HepG2 using 67025-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 67025-1-Ig

    IF Staining of HepG2 using 67025-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67025-1-Ig (DDX5 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNCI-H1299 cells, A431 cells, HSC-T6 cells, HeLa cells, HEK-293 cell, HepG2 cells, HT-29 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67025-1-Ig targets DDX5 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX5 fusion protein Ag24821 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Observed molecular weight 69 kDa
GenBank accession numberNM_004396
Gene symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2882340
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DDX5 antibody 67025-1-IgDownload protocol
IHC protocol for DDX5 antibody 67025-1-IgDownload protocol
IF protocol for DDX5 antibody 67025-1-IgDownload protocol
IP protocol for DDX5 antibody 67025-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Theranostics

LncRNA PRADX-mediated recruitment of PRC2/DDX5 complex suppresses UBXN1 expression and activates NF-κB activity, promoting tumorigenesis.

Authors - Yansheng Li
View Article
humanWB,IP

Cancer Biol Ther

VAMP8 suppresses the metastasis via DDX5/β-catenin signal pathway in osteosarcoma

Authors - Shuo Yang
View Article