CoraLite® Plus 488-conjugated DDX21 Monoclonal antibody
DDX21 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B9C8
Cat no : CL488-66925
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66925-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66925 targets DDX21 in IF, FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DDX21 fusion protein Ag17850 相同性解析による交差性が予測される生物種 |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 |
| Calculated molecular weight | 783 aa, 87 kDa |
| GenBank accession number | BC008071 |
| Gene symbol | DDX21 |
| Gene ID (NCBI) | 9188 |
| RRID | AB_2919389 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DDX21 antibody CL488-66925 | Download protocol |
| FC protocol for CL Plus 488 DDX21 antibody CL488-66925 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
