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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

DDX21 Polyclonal antibody

DDX21 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10528-1-AP

Synonyms

DDX21, DEAD box protein 21, Gu alpha, GUA, GURDB, Nucleolar RNA helicase 2, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/GU, RH II/GuA

Validation Data Gallery

Applications:
  • WB analysis using 10528-1-AP

    WB analysis using 10528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10528-1-AP

    WB analysis using 10528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10528-1-AP

    WB analysis of HeLa using 10528-1-AP

    WB result of DDX21 antibody (10528-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells.

  • WB analysis using 10528-1-AP

    WB analysis using 10528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 10528-1-AP

    IP experiment of COLO 320 using 10528-1-AP

    IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:1000) with COLO 320 cells lysate 2800ug.

  • IHC staining of human colon cancer using 10528-1-AP

    IHC staining of human colon cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10528-1-AP

    IHC staining of human colon cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10528-1-AP

    IHC staining of human breast cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10528-1-AP

    IHC staining of human breast cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10528-1-AP

    IF Staining of HeLa using 10528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 10528-1-AP

    IF Staining of HeLa using 10528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 10528-1-AP

    FC experiment of HepG2 using 10528-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human DDX21 (10528-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells, PC-3 cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10528-1-AP targets DDX21 in WB, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX21 fusion protein Ag0804 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC008071
Gene symbol DDX21
Gene ID (NCBI) 9188
RRIDAB_2092705
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. Catalog# 10528-1-AP is a rabbit polyclonal antibody raised against N-terminal of human DDX21.

Protocols

Product Specific Protocols
WB protocol for DDX21 antibody 10528-1-APDownload protocol
IHC protocol for DDX21 antibody 10528-1-APDownload protocol
IF protocol for DDX21 antibody 10528-1-APDownload protocol
IP protocol for DDX21 antibody 10528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Science

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Authors - Man Wu
View Article
humanWB,IF,chIP

Cell

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Authors - Yu-Hang Xing
  • KO Validated
View Article
humanWB,IHC,IF

Mol Cell

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

Authors - Dae-Seok Kim
View Article
humanIP

Dev Cell

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

Authors - Jeanette A Johansson
View Article
humanWB,IF

Cancer Res

The roles of RNA helicases in DNA damage repair and tumorigenesis reveal precision therapeutic strategies.

Authors - Jinru Xie
  • KD Validated
View Article
humanWB

Mol Cell Proteomics

Differential protein expression profiling by iTRAQ-two-dimensional LC-MS/MS of human bladder cancer EJ138 cells transfected with the metastasis suppressor KiSS-1 gene.

Authors - Ruppen Isabel I
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alexandra (Verified Customer) (09-04-2023)

Very good signal. I would recommend a much higher dilution than 1:200.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: P19 derived neurons
DDX21 Antibody Immunofluorescence validation (1:200 dilution) in P19 derived neurons (Cat no:10528-1-AP)
FH

Wael (Verified Customer) (12-13-2022)

Hek293 fractionated to nuclear and cytoplasmic fractions, DDX21 antibody detects strong band at the expected size of DDX21 and showing nuclear localization as expected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
DDX21 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:10528-1-AP)