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DDX17 Polyclonal antibody
DDX17 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, PLA, IHC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 19910-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293T cells, HEK-293 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| IP | See 5 publications below |
| CoIP | See 2 publications below |
| ChIP | See 3 publications below |
Product Information
19910-1-AP targets DDX17 in WB, IP, IF, PLA, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DDX17 fusion protein Ag13723 相同性解析による交差性が予測される生物種 |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 |
| Calculated molecular weight | 729 aa, 80 kDa |
| Observed molecular weight | 72-80 kDa |
| GenBank accession number | BC000595 |
| Gene symbol | DDX17 |
| Gene ID (NCBI) | 10521 |
| RRID | AB_10667004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. a predicted molecular mass of DDX17 is 72 kDa, but endogenous DDX17 migrates aberrantly at 79 kDa on SDS-PAGE (PMID: 8871553).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX17 antibody 19910-1-AP | Download protocol |
| IHC protocol for DDX17 antibody 19910-1-AP | Download protocol |
| IF protocol for DDX17 antibody 19910-1-AP | Download protocol |
| IP protocol for DDX17 antibody 19910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1. | ||
Nucleic Acids Res The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. | ||
Nucleic Acids Res RNA helicase-dependent gene looping impacts messenger RNA processing
| ||
PLoS Pathog Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production. | ||
J Biol Chem RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression. | ||
iScience Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH N (Verified Customer) (01-28-2023) | Perfect to detect double bands of DDX17.
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