Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, mouse lung tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human kidney tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:600-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
16947-1-AP targets DCTN6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10561 Product name: Recombinant human DCTN6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC013175 Sequence: MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN 相同性解析による交差性が予測される生物種 |
| Full Name | dynactin 6 |
| Calculated molecular weight | 190 aa, 21 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC013175 |
| Gene Symbol | DCTN6 |
| Gene ID (NCBI) | 10671 |
| RRID | AB_2245654 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00399 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| IP protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| WB protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.
| ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Mol Biol Cell Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles. | ||
Neurobiol Aging Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.
| ||
Cell Rep The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection |
