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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

DCTN6 Polyclonal antibody

DCTN6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16947-1-AP

Synonyms

DCTN6, dynactin 6, Dynactin subunit 6, Dynactin subunit p27, Protein WS 3, WS 3, WS3

Validation Data Gallery

Applications:
  • WB analysis using 16947-1-AP

    WB analysis using 16947-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 16947-1-AP

    WB analysis of mouse lung using 16947-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 16947-1-AP

    IP experiment of mouse testis using 16947-1-AP

    IP result of anti-DCTN6 (IP:16947-1-AP, 4ug; Detection:16947-1-AP 1:500) with mouse testis tissue lysate 4800ug.

  • IHC staining of mouse testis using 16947-1-AP

    IHC staining of mouse testis using 16947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16947-1-AP

    IHC staining of mouse testis using 16947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16947-1-AP

    IHC staining of human kidney using 16947-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 16947-1-AP

    IHC staining of human kidney using 16947-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human spleen using 16947-1-AP

    IHC staining of human spleen using 16947-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 16947-1-AP

    IHC staining of human spleen using 16947-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, mouse lung tissue, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16947-1-AP targets DCTN6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCTN6 fusion protein Ag10561 相同性解析による交差性が予測される生物種
Full Name dynactin 6
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC013175
Gene symbol DCTN6
Gene ID (NCBI) 10671
RRIDAB_2245654
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DCTN6 antibody 16947-1-APDownload protocol
IHC protocol for DCTN6 antibody 16947-1-APDownload protocol
IP protocol for DCTN6 antibody 16947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
MonkeyWB

EMBO J

Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.

Authors - Yeh Ting-Yu TY
  • KD Validated
View Article
humanWB

Curr Biol

An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.

Authors - Florencia di Pietro
View Article
humanWB

Mol Biol Cell

Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.

Authors - Samantha L Cavolo
View Article
mouseWB,IF,IP

Neurobiol Aging

Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.

Authors - Md Golam Sharoar
  • KD Validated
View Article