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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

DCTN5 Polyclonal antibody

DCTN5 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 10182-1-AP

Synonyms

DCTN5, dynactin 5 (p25), Dynactin subunit 5, Dynactin subunit p25, p25

Validation Data Gallery

Applications:
  • IHC staining of human breast cancer using 10182-1-AP

    IHC staining of human breast cancer using 10182-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10182-1-AP (DCTN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10182-1-AP

    IHC staining of human breast cancer using 10182-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10182-1-AP (DCTN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10182-1-AP targets DCTN5 in WB, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, Monkey, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCTN5 fusion protein Ag0234 相同性解析による交差性が予測される生物種
Full Name dynactin 5 (p25)
Calculated molecular weight 20 kDa
GenBank accession numberBC004191
Gene symbol DCTN5
Gene ID (NCBI) 84516
RRIDAB_2088344
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for DCTN5 antibody 10182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
MonkeyWB

EMBO J

Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.

Authors - Yeh Ting-Yu TY
View Article
ratWB

J Hazard Mater

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Authors - Yiyuan Kang
View Article
humanWB

Curr Biol

An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.

Authors - Florencia di Pietro
View Article
MonkeyWB

Mol Biol Cell

Dynactin's pointed-end complex is a cargo-targeting module.

Authors - Ting-Yu Yeh
  • KD Validated
View Article
MonkeyWB

Mol Biol Cell

Dynactin integrity depends upon direct binding of dynamitin to Arp1.

Authors - Frances Ka Yan Cheong
View Article
humanIHC,IF

Sci Rep

Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.

Authors - Zhou Yu
View Article