Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10182-1-AP targets DCTN5 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, Monkey, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0234 Product name: Recombinant human DCTN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-182 aa of BC004191 Sequence: ELGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVVPPFTVFSGCPGLFSGELPECTQELMIDVTKSYYQKFLPLTQV 相同性解析による交差性が予測される生物種 |
| Full Name | dynactin 5 (p25) |
| Calculated molecular weight | 20 kDa |
| GenBank accession number | BC004191 |
| Gene Symbol | DCTN5 |
| Gene ID (NCBI) | 84516 |
| RRID | AB_2088344 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTE1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCTN5 antibody 10182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. | ||
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Sci Rep Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions. |
