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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

DCTN4 Polyclonal antibody

DCTN4 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 17098-1-AP

Synonyms

DCTN4, dynactin 4 (p62), Dynactin subunit 4, Dynactin subunit p62

Validation Data Gallery

Applications:
  • WB analysis of human brain using 17098-1-AP

    WB analysis of human brain using 17098-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 17098-1-AP

    WB analysis of Jurkat using 17098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 17098-1-AP

    WB analysis of HepG2 using 17098-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 17098-1-AP

    IP experiment of Jurkat using 17098-1-AP

    IP result of anti-DCTN4 (IP:17098-1-AP, 4ug; Detection:17098-1-AP 1:700) with Jurkat cells lysate 2800ug.

Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

17098-1-AP targets DCTN4 in WB, IP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, Monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCTN4 fusion protein Ag10579 相同性解析による交差性が予測される生物種
Full Name dynactin 4 (p62)
Calculated molecular weight 460 aa, 52 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC026323
Gene symbol DCTN4
Gene ID (NCBI) 51164
RRIDAB_2276944
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DCTN4 antibody 17098-1-APDownload protocol
IP protocol for DCTN4 antibody 17098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
MonkeyWB

Mol Biol Cell

Dynactin's pointed-end complex is a cargo-targeting module.

Authors - Ting-Yu Yeh
  • KD Validated
View Article
humanWB

Onco Targets Ther

Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p.

Authors - Xin Xing Li
View Article