Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HL-60 using 13095-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 13095-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 13095-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13095-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 13095-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 13095-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skin using 13095-1-AP
rat skin tissue were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 13095-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 13095-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 13095-1-AP
IP result of anti-DCT (IP:13095-1-AP, 4ug; Detection:13095-1-AP 1:500) with HL-60 cells lysate 4000ug.
IHC staining of human skin using 13095-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 13095-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A549 using 13095-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (13095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DCT Polyclonal antibody

DCT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13095-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TRP2, TRP 2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, EC:5.3.3.12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue
Positive IP detected in	HL-60 cells
Positive IHC detected in	human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 14 publications below
IHC	See 1 publications below
IF	See 4 publications below

Product Information

13095-1-AP targets DCT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3714 Product name: Recombinant human DCT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC028311 Sequence: MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNA 相同性解析による交差性が予測される生物種
Full Name	dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated molecular weight	519 aa, 59 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC028311
Gene Symbol	DCT
Gene ID (NCBI)	1638
RRID	AB_2090576
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P40126
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Protocols

Product Specific Protocols
IF protocol for DCT antibody 13095-1-AP	Download protocol
IHC protocol for DCT antibody 13095-1-AP	Download protocol
IP protocol for DCT antibody 13095-1-AP	Download protocol
WB protocol for DCT antibody 13095-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Curr Biol Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin. Authors - Ethan D Buhr View Article
human	IHC	Hum Mol Genet Transcriptome analysis and molecular signature of human retinal pigment epithelium. Authors - Strunnikova N V NV View Article
human	WB	J Invest Dermatol miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes. Authors - Chonglin Luo View Article
human	WB	J Cell Mol Med Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes Authors - Yihui Xie View Article
human	WB	Sci Rep Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage. Authors - Sabrina Fehrenbach View Article
mouse	WB	Pharmaceutics Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion. Authors - Xudong Tang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HL-60 using 13095-1-AP

WB analysis of A375 using 13095-1-AP

WB analysis of A431 using 13095-1-AP

WB analysis of MCF-7 using 13095-1-AP

WB analysis of HL-60 using 13095-1-AP

WB analysis of A431 using 13095-1-AP

WB analysis of rat skin using 13095-1-AP

WB analysis of COS-7 using 13095-1-AP

WB analysis of HL-60 using 13095-1-AP

IP experiment of HL-60 using 13095-1-AP

IHC staining of human skin using 13095-1-AP

IHC staining of human skin using 13095-1-AP

IF Staining of A549 using 13095-1-AP

DCT Polyclonal antibody

DCT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes.

Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes

Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage.

Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion.