• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22373

Synonyms

DCP1A, FLJ21691, HSA275986, mRNA decapping enzyme 1A, Nbla00360, SMAD4IP1, SMIF, Transcription factor SMIF

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-22373

    IF Staining of HepG2 using CL488-22373

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DCP1A antibody (CL488-22373) at dilution of 1:100.

  • FC experiment of HeLa using CL488-22373

    FC experiment of HeLa using CL488-22373

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DCP1A (CL488-22373) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22373-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-22373 targets DCP1A in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCP1A fusion protein Ag18027 相同性解析による交差性が予測される生物種
Full Name DCP1 decapping enzyme homolog A (S. cerevisiae)
Calculated molecular weight 582 aa, 63 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007439
Gene symbol DCP1A
Gene ID (NCBI) 55802
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DCP1A antibody CL488-22373Download protocol
FC protocol for CL Plus 488 DCP1A antibody CL488-22373Download protocol
Standard Protocols
Click here to view our Standard Protocols