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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCAR2 Polyclonal antibody

CCAR2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22638-1-AP

Synonyms

ccar2, DBC.1, DBC1, DBC-1, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, Protein KIAA1967

Validation Data Gallery

Applications:
  • WB analysis using 22638-1-AP

    WB analysis using 22638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 22638-1-AP

    WB analysis of HeLa using 22638-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 22638-1-AP

    WB analysis of HEK-293 using 22638-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 22638-1-AP

    WB analysis of mouse brain using 22638-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 22638-1-AP

    WB analysis of rat brain using 22638-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 22638-1-AP

    WB analysis of MCF-7 using 22638-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 22638-1-AP

    IP experiment of mouse brain using 22638-1-AP

    IP result of anti-CCAR2 (IP:22638-1-AP, 4ug; Detection:22638-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human breast cancer using 22638-1-AP

    IHC staining of human breast cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human breast cancer using 22638-1-AP

    IHC staining of human breast cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human breast cancer using 22638-1-AP

    IHC staining of human breast cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:100.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human breast cancer using 22638-1-AP

    IHC staining of human breast cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:100.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human colon cancer using 22638-1-AP

    IHC staining of human colon cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human colon cancer using 22638-1-AP

    IHC staining of human colon cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human pancreas cancer using 22638-1-AP

    IHC staining of human pancreas cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human pancreas cancer using 22638-1-AP

    IHC staining of human pancreas cancer using 22638-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IF Staining of HepG2 using 22638-1-AP

    IF Staining of HepG2 using 22638-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA1967 antibody 22638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DBC1/CCAR2 Ready-To-Use IHC Kit. DBC1/CCAR2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22638-1-AP is CCAR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCAR2 fusion protein Ag18374 相同性解析による交差性が予測される生物種
Full Name KIAA1967
Calculated molecular weight 923 aa, 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC018269
Gene symbol KIAA1967
Gene ID (NCBI) 57805
RRIDAB_2879138
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCAR2 antibody 22638-1-APDownload protocol
IHC protocol for CCAR2 antibody 22638-1-APDownload protocol
IF protocol for CCAR2 antibody 22638-1-APDownload protocol
IP protocol for CCAR2 antibody 22638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP,RIP

Nucleic Acids Res

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.

Authors - Ruibing Chen
View Article
mouseWB, IF

Biochem Cell Biol

Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.

Authors - Farshad Niri
View Article
humanWB,IHC,IP

Elife

Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression

Authors - Qiangqiang Liu
View Article