Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 14876-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14876-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14876-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14876-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14876-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14876-1-AP
IP result of anti-DAAM1 (IP:14876-1-AP, 3ug; Detection:14876-1-AP 1:500) with mouse liver tissue lysate 8000ug.
IHC staining of human brain using 14876-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14876-1-AP (DAAM1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 14876-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14876-1-AP (DAAM1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 14876-1-AP
Immunofluorescent analysis of HepG2 cells, using DAAM1 antibody 14876-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

DAAM1 Polyclonal antibody

DAAM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14876-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

KIAA0666, FLJ41657, Disheveled-associated activator of morphogenesis 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, mouse heart tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 21 publications below
IHC	See 11 publications below
IF	See 10 publications below
CoIP	See 1 publications below

Product Information

14876-1-AP targets DAAM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, canine, goat, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6648 Product name: Recombinant human DAAM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 715-1068 aa of BC064999 Sequence: KDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF 相同性解析による交差性が予測される生物種
Full Name	dishevelled associated activator of morphogenesis 1
Calculated molecular weight	123 kDa
Observed molecular weight	123 kDa
GenBank accession number	BC064999
Gene Symbol	DAAM1
Gene ID (NCBI)	23002
RRID	AB_2089444
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4D1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

Protocols

Product Specific Protocols
IF protocol for DAAM1 antibody 14876-1-AP	Download protocol
IHC protocol for DAAM1 antibody 14876-1-AP	Download protocol
IP protocol for DAAM1 antibody 14876-1-AP	Download protocol
WB protocol for DAAM1 antibody 14876-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Signal Transduct Target Ther C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness Authors - Jun Ma View Article
human	WB,IF	J Cell Biol DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility. Authors - Tamako Nishimura KD Validated View Article
mouse	WB,IF	Ecotoxicol Environ Saf Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny Authors - Hua Du View Article
mouse	WB,IHC	Development Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis. Authors - Li Deqiang D KO Validated View Article
human	WB,IF,IHC	Cell Prolif Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer. Authors - Leiyu Hao View Article
human	WB	Cell Signal miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer. Authors - Huaping Xiong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 14876-1-AP

WB analysis of HepG2 using 14876-1-AP

WB analysis of human heart using 14876-1-AP

WB analysis of mouse liver using 14876-1-AP

WB analysis of mouse heart using 14876-1-AP

IP experiment of mouse liver using 14876-1-AP

IHC staining of human brain using 14876-1-AP

IHC staining of human brain using 14876-1-AP

IF Staining of HepG2 using 14876-1-AP

DAAM1 Polyclonal antibody

DAAM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness

DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.

Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny

Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.

Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.

miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer.