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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 8-specific Polyclonal antibody

Cytokeratin 8-specific Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27105-1-AP

Synonyms

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytokeratin 8-specific, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

Validation Data Gallery

Applications:
  • WB analysis using 27105-1-AP

    WB analysis using 27105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27105-1-AP

    IHC staining of human tonsillitis using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of human tonsillitis using 27105-1-AP

    IHC staining of human tonsillitis using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of rat skin using 27105-1-AP

    IHC staining of rat skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of rat skin using 27105-1-AP

    IHC staining of rat skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of mouse skin using 27105-1-AP

    IHC staining of mouse skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of mouse skin using 27105-1-AP

    IHC staining of mouse skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of human colon using 27105-1-AP

    IHC staining of human colon using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of human colon using 27105-1-AP

    IHC staining of human colon using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IF Staining of HepG2 using 27105-1-AP

    IF Staining of HepG2 using 27105-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 8-specific antibody (27105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27105-1-AP is Cytokeratin 8-specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 8-specific fusion protein Ag25844 相同性解析による交差性が予測される生物種
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight52 kDa
GenBank accession numberBC008200
Gene symbol KRT8
Gene ID (NCBI) 3856
RRIDAB_2918117
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8-specific antibody 27105-1-APDownload protocol
IHC protocol for Cytokeratin 8-specific antibody 27105-1-APDownload protocol
IF protocol for Cytokeratin 8-specific antibody 27105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Lung Cancer Res

Identification of lysosomal genes associated with prognosis in lung adenocarcinoma

Authors - Houqiang Li
View Article
humanIHC

Environ Toxicol

Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer

Authors - Hua Chen
View Article