Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 28603-1-AP
WB result of Cyclin B1 antibody (28603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
WB analysis using 28603-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28603-1-AP (Cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 28603-1-AP
IP result of anti-Cyclin B1 (IP:28603-1-AP, 4ug; Detection:28603-1-AP 1:2000) with HeLa cells lysate 1800 ug.
IHC staining of human skin cancer using 28603-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 28603-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 28603-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 28603-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 28603-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HT-29 using 28603-1-AP
Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 28603-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of Ramos using 28603-1-AP
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human Cyclin B1 (28603-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 28603-1-AP

Publications(83)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (83)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, HeLa cells, K-562 cells, C6 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HT-29 cells
Positive FC (Intra) detected in	Ramos cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 78 publications below
IHC	See 6 publications below
IF	See 3 publications below

Product Information

28603-1-AP targets Cyclin B1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, rat, pig, canine, chicken, bovine, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag29426 Product name: Recombinant human CCNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC006510 Sequence: MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEE 相同性解析による交差性が予測される生物種
Full Name	cyclin B1
Calculated molecular weight	48 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC006510
Gene Symbol	Cyclin B1
Gene ID (NCBI)	891
RRID	AB_2881179
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14635
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
IF protocol for Cyclin B1 antibody 28603-1-AP	Download protocol
IHC protocol for Cyclin B1 antibody 28603-1-AP	Download protocol
IP protocol for Cyclin B1 antibody 28603-1-AP	Download protocol
WB protocol for Cyclin B1 antibody 28603-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins Authors - Yulin Chen View Article
human	WB	Clin Transl Med Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes Authors - Jian Chen View Article
human	WB	Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. Authors - Xintian Chen View Article
human	WB	Mol Oncol Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma Authors - Mayu Nakagawa View Article
human	WB	Life Sci Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway Authors - Jia-Peng Lin View Article
rat,human	WB,IHC	Am J Physiol Lung Cell Mol Physiol KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension Authors - Xiaochen Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 28603-1-AP

WB analysis using 28603-1-AP

IP experiment of HeLa using 28603-1-AP

IHC staining of human skin cancer using 28603-1-AP

IHC staining of human skin cancer using 28603-1-AP

IHC staining of human tonsillitis using 28603-1-AP

IHC staining of human tonsillitis using 28603-1-AP

IF Staining of HeLa using 28603-1-AP

IF Staining of HT-29 using 28603-1-AP

IF Staining of HeLa using 28603-1-AP

FC experiment of Ramos using 28603-1-AP

Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes

Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.

Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma

Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway

KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension