Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-1080 cells, mouse colon tissue, HeLa cells, pig colon tissue, rat colon tissue, Saos-2 cells, SiHa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 5 publications below |
| IF | See 3 publications below |
Product Information
67604-1-Ig targets COL5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type V, alpha 1 |
| Calculated molecular weight | 184 kDa |
| Observed molecular weight | 220 kDa |
| GenBank accession number | NM_000093 |
| Gene Symbol | COL5A1 |
| Gene ID (NCBI) | 1289 |
| RRID | AB_2882810 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20908 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
| IHC protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
| IF protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. | ||
Exp Ther Med COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis | ||
Neuropathology Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains. | ||
Comput Biol Med Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma | ||
J Adv Res Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4 | ||
Cell Signal Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT |