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Catalase 抗体 4 文献

Mouse Monoclonal| カタログ番号: 66765-1-Ig |クローン番号: 1F8D7

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適用アプリケーション

アプリケーション:
WB, IHC, IF, ELISA
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Images See all

Catalase Antibody WB HeLa cells 66765-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Catalase Antibody IHC human hepatocirrhosis tissue 66765-1-Ig

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 10x lens).

Catalase Antibody IHC human hepatocirrhosis tissue 66765-1-Ig

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 40x lens).

Catalase Antibody IF HepG2 cells 66765-1-Ig

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66765-1-Ig (Catalase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

種特異性:
Human, Mouse, Rat, Pig
WB ポジティブ:
HeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue
IHC ポジティブ:
human hepatocirrhosis tissue
IF ポジティブ:
HepG2 cells
推奨希釈倍率:
WB : 1:2000-1:10000
IHC : 1:100-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein G purification
アイソタイプ:
IgG1
バッファー/保存方法:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Catalase fusion protein Ag16916
正式名:
catalase
計算分子量:
60 kDa
検出分子量:
60 kDa
GenBank accession number:
BC112219
Gene ID (NCBI):
847
遺伝子シンボル:
CAT
別名:
CAT, catalase

背景説明

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.


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