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KD/KO Validated

Caspase 7/p20 Polyclonal antibody

Name: Caspase 7/p20 Polyclonal antibody
Brand: Proteintech
SKU: 27155-1-AP

Caspase 7/p20 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27155-1-AP

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase 7/p20, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

Validation Data Gallery

Applications:

AllWBIPIF

WB analysis using 27155-1-AP
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 27155-1-AP
WB result of Caspase 7 antibody (27155-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 7 transfected HEK-293 cells.
WB analysis of HEK-293 using 27155-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 27155-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 27155-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 27155-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 27155-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 27155-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 27155-1-AP
IP result of anti-Caspase 7/p20 (IP:27155-1-AP, 4ug; Detection:27155-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.
IF Staining of MCF-7 using 27155-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 27155-1-AP (Caspase 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 27155-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7/p20 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 27155-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7/p20 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 27155-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells, mouse liver tissue, PC-3 cells
Positive IP detected in	HEK-293 cells
Positive IF detected in	MCF-7 cells, mouse brain tissue

Planning an IHC experiment? We recommend our IHCeasy CASP7 Ready-To-Use IHC Kit. CASP7 primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 48 publications below
IHC	See 2 publications below
IP	See 1 publications below

Product Information

27155-1-AP targets Caspase 7/p20 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 7/p20 fusion protein Ag25904 相同性解析による交差性が予測される生物種
Full Name	caspase 7, apoptosis-related cysteine peptidase
Calculated molecular weight	303 aa, 34 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC015799
Gene symbol	CASP7
Gene ID (NCBI)	840
RRID	AB_2880779
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Protocols

Product Specific Protocols
WB protocol for Caspase 7/p20 antibody 27155-1-AP	Download protocol
IF protocol for Caspase 7/p20 antibody 27155-1-AP	Download protocol
IP protocol for Caspase 7/p20 antibody 27155-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model Authors - Mengyan Hu View Article
human	WB	J Pineal Res Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways. Authors - Yue Gao View Article
human	WB	Cell Rep Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation Authors - Zehe Ge View Article
human	WB	Front Immunol Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma Authors - Jinfeng Zhu View Article
human	WB	Phytother Res Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT Authors - Xiaoli Li View Article
human	WB	J Agric Food Chem β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway. Authors - Heshan Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caspase 7/p20 Polyclonal antibody

Caspase 7/p20 Polyclonal Antibody for IF, IP, WB, ELISA

Validation Data Gallery

WB analysis using 27155-1-AP

WB analysis of HEK-293 using 27155-1-AP

WB analysis of HEK-293 using 27155-1-AP

WB analysis of LNCaP using 27155-1-AP

WB analysis of LNCaP using 27155-1-AP

WB analysis of MCF-7 using 27155-1-AP

WB analysis of PC-3 using 27155-1-AP

WB analysis of mouse liver using 27155-1-AP

IP experiment of HEK-293 using 27155-1-AP

IF Staining of MCF-7 using 27155-1-AP

IF Staining of mouse brain using 27155-1-AP

IF Staining of mouse brain using 27155-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation

Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma

Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT

β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.