CoraLite® Plus 488-conjugated Cardiac Troponin T Monoclonal antibody

Cardiac Troponin T Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, rabbit, pig, chicken

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E2D8

Cat no : CL488-68300

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle



Tested Applications

Positive IF detected inH9C2 cells
For other applications, we recommend the unconjugated version of this antibody, 68300-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-68300 targets Cardiac Troponin T in IF applications and shows reactivity with mouse, rat, rabbit, pig, chicken samples.

Tested Reactivity mouse, rat, rabbit, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag7716 相同性解析による交差性が予測される生物種
Full Name troponin T type 2 (cardiac)
Calculated molecular weight 36 kDa
Observed molecular weight34-40 kDa
GenBank accession numberBC002653
Gene symbol TNNT2
Gene ID (NCBI) 7139
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cardiac Troponin T antibody CL488-68300Download protocol
Standard Protocols
Click here to view our Standard Protocols